Cas12 (LbCpf1), Active (C12CR-E241H)

Cas12 (LbCpf1), Active (C12CR-E241H)

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Description :Recombinant Lachnospiraceae Cas12 (LbCpf1) (1-1227) was expressed in E. coli with a C-terminal His tag

Species :Lachnospiraceae

Tag : HIS tag

Expression System: E.coli

Sequence :1-1227

Genbank Number :ATB19154

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Activity: Sample Purity Data. For specific information on a given lot, see the related technical datasheet.

Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight : 130 kDa(calculated MW ~140 kDa)

Gene Aliases :Cpf1 (synthetic construct)

Scientific Background :CRISPR (clustered regularly interspaced short palindromic repeat) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development (3). Developing rapid and sensitive diagnostic tools is key for successful patient management and curbing the disease spread. Several CRISPR-Cas12 based SARS-CoV-2 detection assays have been developed (4,5). CRISPR/Cas12 (LbCpf1) is a Cas12 synthetic construct derived from Lachnospiraceae bacterium. 

References :

  1. Horvath, P., et al. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
  2. Morange, M. What history tells us XXXVII.  CRISPR-Cas: The disconvery of an immune system in prokaryotes.  Biosci. 40(2):221-223.
  3. Gier, R.A., et al. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 2020, 11(1):1-9.
  4. Broughton JP, et al: Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR-based DETECTR lateral flow assay. medRxiv doi: https://doi.org/10.1101/ 03.06.20032334.
  5. Lucia C, et al: An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12. bioRxiv. doi: https://doi.org/10.1101/ 02.29.971127.

Product Sheets (By Lot #) :

N3617-7