• $270.00


Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the c-JUN protein

Cited Applications :WB

Cross Reactivity :Western blot human and mouse c-JUN

c-JUN from other species may also be detectable.

Host :Rabbit

Immunogen :Synthetic peptide corresponding to amino acids 71-75 of human c-JUN

Purification :Affinity Chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative Western blot with Anti-c-Jun (1:1000) using 15ng of GST-tagged human recombinant c-JUN.

Sample Data : Representative Western blot with Anti-c-JUN (1:1000) using 20μg, 40μg and 65μg of 3T6 cell lysate.

Scientific Background :c-Jun is a proto-oncogene that forms a complex with c-Fos which regulates transcription from promoters containing AP-1 activation elements (1). c-Jun has specific DNA binding activity and following in vitro translation, c-Jun binds as a homodimer to the AP-1 DNA site. The transactivating function of c-Jun is acutely regulated by a wide variety of cellular signals via modulation of phosphorylation of two serines (63 and 73). Jun N-terminal domain kinases (JNKs), are responsible for mediating S63/73 phosphorylation on c-Jun in response to a variety of cellular stimuli including TNF-a, heat stress and u.v. light (2).

References :
1.Halazonetis, T D. et al: c-Jun dimerizes with itself and with c-Fos, forming complexes of different DNA binding affinitie. Cell. 1988 Dec 2; 55(5):917-24.

2.Dai, T. et al: Stress-activated protein kinases bind directly to the delta domain of c-Jun in resting cells: implications for repression of c-Jun function. Oncogene. 1995 Mar 2; 10(5):849-55.

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Research Areas :Cancer, Cytoplasmic Tyrosine Kinases, Inflammation, Neurobiology, Cancer, Neurobiology, Inflammation, Cytoplasmic Tyrosine Kinases