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Description :Recombinant full-length human BRD3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_007371
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :115 kDa
Gene Aliases :KIAA0043; ORFX; RING3L
Scientific Background :As the best characterized binding motif for lysine acetylation, Bromodomains (BRDs) are 110 amino acid highly conserved modules found in transcriptional co-regulators, chromatin modifying enzymes and nuclear scaffold proteins. Initially identified in the protein encoded by the D. melanogaster brahama gene. Along with BRD2, BRD4 and BRDT, Bromodomain-containing protein 3 (BRD3) lacks intrinsic chromatin modifying potential and primarily function as scaffolds for transcription factors or other chromatin regulating factors. It is characterized by the presence of two distinct BRDs at the N-terminal end of the protein. BRD3 is involved in the pathognomonic chromosomal translocations seen in NUT midline carcinoma (NMC).
1. French CA, et al: BRD4-NUT fusion oncogene: a novel mechanism in aggressive carcinoma. Cancer Res. 63(2):304-7, 2003.
2. Barbieri I, et al: Bromodomains as therapeutic targets in cancer. Brief Funct Genomics. 12(3):219-30, 2013.
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Research Areas :Cancer, Cardiovascular Disease, Cell Cycle, Inflammation, Neurobiology