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Beta-Defensin 3 (DEFB103A)(D823-40N)

Beta-Defensin 3 (DEFB103A)(D823-40N)

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Description :Recombinant Human Beta-Defensin 3 (DEFB103A) was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :P81534

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight :5.4 kDa

Gene Aliases :Beta-defensin 3, BD-3, DEFB-3, HBD3, hBD-3, Defensin, beta 103, Defensin-like protein, BD3, DEFB103, DEFB3.

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :Defensins are a large family of peptides of which two groups exist in mammals: alpha defensins and beta defensins - which are distinguishable by the spacing and connectivity of the conserved cysteine residues within the mature peptides. It is thought that the function of defensins in the eradication of pathogens from the host system is to insert themselves into the bacterial membrane under the influence of membrane potential, in doing so form channels which lead to leakage of cytoplasmic molecules and cell death. Unlike hBD-1, this second peptide, human beta-defensin-2 (hBD-2), is regulated at a transcriptional level in response to contact with microorganisms, and is highly effective in killing the gram negative bacteria Its expression is also upregulated by the cytokine tumor necrosis factor-alpha.

Sample Cell Assay Data :The ED50 as determined by its antimicrobial resistance to E.coli was determined to be < 3.0 ug/mL.

Purity :Recombinant Human Beta-Defensin 3 (DEFB103A) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brialliant Blue G-250.

Product Sheets (By Lot #) :

G3211-9.pdf

Research Areas :Cancer