beta-Actin Positive Control siRNA
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Description : beta-Actin Positive Control siRNA is a synthetic siRNA duplex designed to knock-down human beta-Actin mRNA expression. The synthetic siRNAs (5’-UGA AGA UCA AGA UCA UUG CdTdT-3’ and 5’-GCA AUG AUC UUG AUC UUC AdTdT-3’) enable qualitative assessment of knockdown and for optimizing RNAi experiments.
Specificity : beta-Actin Positive Control siRNA is designed to specifically knock-down human beta-Actin expression.
Purity : Single PAGE band
Reconstitution Protocol : Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Format : Lyophilized powder
Storage and Stability : The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
Scientific Background : Actin, a ubiquitous protein in eukaryotes, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle beta- and gamma-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controling cell structure and motility (1). alpha-cardiac and alpha-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, alpha- and gamma-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells (1).
1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48–55.
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Research Areas: Cancer, Invasion/Metastasis,