Anti-BID(B59-363R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the BID protein
Cited Applications :WB, ELISA, IF, IHC
Cross Reactivity :Human and Mouse
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :BID antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human BID
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of BID in mouse lung cell lysates with BID antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Sample Data :Immunohistochemical staining of mouse lung tissue using BID antibody at 2 ug/ml.
Scientific Background :BID is a BH3 interacting death domain that heterodimerizes with either agonist BAX or antagonist BCL2 (1). BID is a member of the BCL-2 family of cell death regulators and is a mediator of mitochondrial damage induced by caspase-8 (CASP8). BID initiates apoptosis by binding to regulatory sites on prosurvival BCL2 proteins to directly neutralize their function. Multiple alternatively spliced transcript variants of BID have been found, but the full-length nature of some variants has not been defined. BID together with Cathepsins play an important role in the actions of Camptothecin on breast cancer cells (2).
References :
1. Hayakawa, A. et al:, Bid truncation mediated by caspases-3 and -9 in vinorelbine-induced apoptosis. Apoptosis. 2008;13(4):523-30.
2. Lamparska-Przybysz. M. et al: Cathepsins and BID are involved in the molecular switch between apoptosis and autophagy in breast cancer MCF-7 cells exposed to camptothecin. J Physiol Pharmacol. 2005 Jun;56 Suppl 3:159-79.
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Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Neurobiology, Cancer, Neurobiology, Cardiovascular Disease, Apoptosis/Autophagy