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Description :Rabbit Polyclonal Antibody

Species :Rabbit

Tag :

Expression System:

Sequence :

Specificity :Recognizes the BCL2 protein

Cited Applications :WB, IP

Cross Reactivity :Immunoprecipitation of BCL2 from human cells

Western blot of BCL2 from human cells.

BCL2 from other species may also be detectable.

Host :Rabbit

Immunogen :Synthetic peptide corresponding to amino acids 54-58 of human BCL2

Purification :Affinity chromatography

Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Immunoprecipitation :Representative immunoprecipitation with 2 μg of Anti-BCL2 using 500 μg of HeLa, HEK-293T, and Jurkat cell lysate.

Sample Western Blot :Representative western blot with Anti-BCL2 (1:1000) using 20 ng of GST tagged human recombinant BCL2 (left image) or 3 μg, 10 μg, and 30 μg of Jurkat cell lysate..

Scientific Background :BCL2 gene encodes an integral inner mitochondrial membrane protein that acts as an antiapoptotic protein (1). The protein BAD can antagonize both the cell cycle and antiapoptotic functions of BCL2 through binding to the BH3 domain. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma (2). BCL2 is phosphorylated on specific serine/threonine residues within the unstructured loop in response to diverse stimuli and such phosphorylation has been associated with the loss of the biological function of BCL2.

References :
1. Hockenbery, D. et al: Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336, 1990.

2. Tsujimoto, Y. et al: Involvement of the bcl-2 gene in human follicular lymphoma. Science 228: 1440-1443, 1985.

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Research Areas :Cancer, Neurobiology, Cardiovascular Disease, Apoptosis/Autophagy, Cancer, Neurobiology, Cardiovascular Disease, Apoptosis/Autophagy