BUB1 Protein(B11-35G)

BUB1 Protein(B11-35G)

  • $215.00


Description :Recombinant human BUB1(756-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :756-end

Genbank Number :NM_004336

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Western Blot

Molecular Weight :~63 kDa

Gene Aliases :BUB1A, BUB1L, hBUB1

Scientific Background :BUB1 is a kinase which involved in spindle checkpoint function that in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. BUB1 is highly expreesed in thymus, ovary, and testis (1). BUB1 maintains the steady-state level and centromeric localization of SGO1, and they concluded that BUB1 protects centromeric cohesion in mitosis through SGO1 (2). BUB1-mediated phosphorylation of CDC20 is required for proper checkpoint signaling and that inhibition of APC by BUB1 may partly account for the sensitivity of the spindle checkpoint. BUB1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes.

References :
1. Leland, S.et.al: Heterozygosity for a Bub1 mutation causes female-specific germ cell aneuploidy in mice. Proc. Nat. Acad. Sci. 106: 12776-12781, 2009.

2. Tang, Z.et.al: Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis. Proc. Nat. Acad. Sci. 101: 18012-18017, 2004.

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Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases