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ANXA4-PKN1 (Aex2Pex13), Active (P70-19G)

ANXA4-PKN1 (Aex2Pex13), Active (P70-19G)

  • $259.00


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Description :Recombinant human fusion protein ANXA4 (1-3a.a., exons1-2)-PKN1 (578a.a.-end, exons13-22) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :1-3a.a., exons1-2 (ANXA4) - 578a.a.-end, exons13-22 (PKN1)

Genbank Number 1:NM_001153

Genbank Number 2:NM_002741

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay

Molecular Weight : 72 kDa

Gene Aliases :ANX4; P32.5; PIG28; PP4-X; ZAP36; PAP-II; HEL-S-274;
PRK1, DBK, PKN1, PKN, MGC46204, PAK1, PRKCL1, PKC-L1

Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol

Scientific Background :PKN1/PRK1 belongs to the protein kinase C superfamily which is activated by Rho family of small G proteins. PKN1/PRK1 is known to mediate the Rho-dependent signaling pathway and it can be activated by phospholipids and by limited proteolysis (1). PDPK1/PDK may also mediate insulin signals to the actin cytoskeleton and the proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggest its role in signal transduction related to apoptosis. PKN1/PRK signaling stimulates AR activity in the presence of adrenal androgens and in the presence of an AR antagonist (2).

References :
1. Amano, M.et.al: Identification of a putative target for rho as the serine-threonine kinase protein kinase N. Science 271: 648-651, 1996.

2. Metzger, E. et.al: A novel inducible transactivation domain in the androgen receptor: implications for PRK in prostate cancer. EMBO J. 22: 270-280, 2003.

Product Sheets (By Lot #) :

M2634-11.pdf

Research Areas :Cancer