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Description :Mouse Monoclonal Antibody
Specificity :Anti-V5 antibody recognizes the N-terminal or C-terminal V5-tagged (GKPIPNPLLGLDST sequence) fusion protein.
Cited Applications :WB, ELISA, IP, IS
Cross Reactivity :All proteins tagged with V5
Host / Isotype / Clone# :Mouse, IgG1, V4RR
Immunogen :GKPIPNPLLGLDST peptide conjugated to KLH
Purification :Purified by Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot (1:1000) using in a positive cell lysate that contains V5 tagged fusion protein.
Scientific Background :The V5 epitope tag (GKPIPNPLLGLDST) consists of 14 highly charged amino acid residues originating from the C-terminus end of the P and V proteins of the simian virus 5, of the paramyxovirus family . This tag is widely employed because of the highly specific antibodies that can be generated against it .
1. Southern, J.A. et al., Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics, J. Gen. Virol. 72, 1991; (7), pp. 1551–1557.
2. Thomas, M, et al., Baculovirus expression of HCoV-OC43 nucleocapsid protein and development of a Western blot assay for detection of human antibodies against HCoV-OC43, J. of Virol. Methods Vol. 139, (2), Feb. 2007; pp. 175-180.
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