Anti-SIRT5(S39-463R)

Anti-SIRT5(S39-463R)

  • $325.00


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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the SIRT5 protein

Cited Applications :WB, ELISA

Cross Reactivity :Human, Mouse and Rat

Host / Isotype / Clone# :Rabbit, IgG

Immunogen :SIRT5 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SIRT5

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot analysis of SIRT5 in human liver tissue lysate with SIRT5 antibody at 1 ug/ml.

Scientific Background :SIRT5 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT5 being a member of class III. SIRT5 consists of eight exons and is found in two isoforms which encode a 310 aa and a 299 aa protein, respectively. Human SIRT5 is most predominantly expressed in heart muscle cells and in lymphoblasts. Fluorescence in situ hybridization analysis localized the human SIRT5 gene to chromosome 6p23. SIRT5 can deacetylate cytochrome c, a protein of the mitochondrial intermembrane space with a central function in oxidative metabolism as well as apoptosis initiation (1).

References :
1. Schlicker C. et al: Substrates and regulation mechanisms for the human mitochondrial sirtuins Sirt3 and Sirt5. J Mol Biol. 2008 Oct 10;382(3):790-801.

2. Mahlknecht, U. et al: Assignment of the NAD-dependent deacetylase sirtuin 5 gene (SIRT5) to human chromosome band 6p23 by in situ hybridization. Cytogenet Genome Res. 2006;112(3-4):208-12.

Product Sheets (By Lot #) :

O2121-36.pdf

Research Areas :Cancer, Cell Cycle, Inflammation, Cancer, Inflammation, Cell Cycle