Anti-SIK3(S12-63R)

Anti-SIK3(S12-63R)

  • $325.00


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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the SIK3 protein

Cited Applications :WB, ELISA, IF

Cross Reactivity :Human, Mouse and Rat

Host / Isotype / Clone# :Rabbit, IgG

Immunogen :SIK3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SIK3

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Sample Data :Immunofluorescence of SIK3 in rat brain cells with SIK3 antibody at 20 ug/ml.

Scientific Background :SIK3 (QSK) is a serine/threonine-protein kinase, belongs to QIK subfamily. The phosphorylation of SIK3 by LKB1 through the 14-3-3 binding enhances its catalytic activity and leads its localization to punctate structures within the cytoplasm (1). Overexpression of SIK3 promotes G1/S cell cycle progression with ovarian cancer (2). There are two sites (H331L and A1103V) were mutated at significant frequency in breast cancer (3). SIK3 is a novel tumor-associated antigen (TAA).

References :
1. Al-Hakim A.K., et.al: 14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK. J. Cell Sci. 118:5661-5673(2005).

2. Charoenfuprasert S, et.al: Identification of salt-inducible kinase 3 as a novel tumor antigen associated with tumorigenesis of ovarian cancer. Oncogene. 2011 Aug 18;30(33):3570-84.

3. Sjoeblom T. et.al: The consensus coding sequences of human breast and colorectal cancers. Science 314:268-274(2006).

Product Sheets (By Lot #) :

O2121-46.pdf

Research Areas :Cancer, Ser/Thr Kinases, Cancer, Ser/Thr Kinases