Anti-ROCK1(R10-63R)

Anti-ROCK1(R10-63R)

  • $325.00


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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the ROCK1 protein and will not cross-react with other ROCK family members

Cited Applications :WB, ELISA, ICC, IF

Cross Reactivity :Human, Mouse and Rat

Host / Isotype / Clone# :Rabbit, IgG

Immunogen :ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Sample Data :Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 ug/ml.

Scientific Background :ROCK1 is a ubiquitously expressed serine/threonine kinase that is a downstream target of the small GTPase RhoA. ROCK1 is involved in diverse cellular functions, including smooth muscle contraction, actin cytoskeleton organization, cell adhesion and motility, and gene expression (1). ROCK1 contributes to the development of cardiac fibrosis and induction of fibrogenic cytokines in cardiomyocytes in response to pathological stimuli. ROCK1 knockout mice exhibit reduced perivascular and interstitial fibrosis which is associated with reduced expression of a variety of extracellular matrix (ECM) proteins and fibrogenic cytokines (2).

References :
1. Zhao, Y M. et al: Rho-associated kinases play a role in endocardial cell differentiation and migration. Dev Biol. 2004 Nov 1;275(1):183-91.

2. Zhang, C. et al: Targeted deletion of ROCK1 protects the heart against pressure overload by inhibiting reactive fibrosis. FASEB J. 2006 May; 20(7):916-25.

Product Sheets (By Lot #) :

O2121-41.pdf

Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Ser/Thr Kinases, Cancer, Inflammation, Cardiovascular Disease, Apoptosis/Autophagy, Invasion/Metastasis, Ser/Thr Kinases