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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the PKCδ protein
Cited Applications :WB
Cross Reactivity :Western blot human PKCd
PKCd from other species may also be detectable
Immunogen :Synthetic peptide corresponding to amino acids 643-647 of human PKCδ
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti- PKCδ (1:1000) using 20ng of GST-tagged human recombinant PKCα, PKCβI, PKCβII, PKCγ, PKCδ, PKCε, PKCη, PKCθ, PKCμ, PKCν, PKCζ, and PKCι.
Scientific Background :Protein kinase C delta (PKCδ) is a member of the protein kinase C (PKC) family of serine-threoninekinases. It is a 79kD protein kinase that shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+ (1). Phosphatidylinositol is the most potent activator of PKCdelta. Northern blot analysis indicated that PKCdelta is widely distributed in almost all the tissues and is a major isoform of PKC expressed in hemopoietic cells (2). PKCdelta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters.
1. Leibersperger, H. et al: Immunological demonstration of a calcium-unresponsive protein kinase C of the delta-type in different species and murine tissues. Predominance in epidermis. J Biol Chem. 1991 Aug 5; 266(22):14778-84.
2. Mischak, H. et al: Mouse protein kinase C-delta, the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the protein. Biochemistry. 1991 Aug 13; 30(32):7925-31.
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Research Areas :Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy, Invasion/Metastasis