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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes peptides and proteins phosphorylated on threonine residues.
Cited Applications :WB, ELISA, IHC, IP
Cross Reactivity :Pan-specific antibody. No reaction to phosphotyrosine peptides and proteins.
Immunogen :Phosphothreonine peptide-KLH conjugates
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot of the phosphthreonine protein profile with Anti-Phosphothreonine (1:500) using mouse spleen extract (A) and sodium vanadate-treated mouse spleen extract (B).
Scientific Background :Post-translational modification of proteins and peptides is a robust way to regulate function of existing proteins or peptides. Protein phosphorylation represents one the most abundant and important post-translational modifications of proteins. Phosphorylation on threonine residues in proteins is performed by a variety of threonine specific protein kinases in the cell which are involved in a wide variety of signaling networks. The Phospho-Threonine antibody detects phosphorylation on threonine residues in proteins and peptides.
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Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, JAK/STAT Pathway, JNK/SAPK Pathway, Metabolic Disorder, Neurobiology, NfkB Pathway, p38 Pathway, PKA/PKC Pathway, WNT Signaling, Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, ERK/MAPK Pathway, p38 Pathway, JNK/SAPK Pathway, NfkB Pathway, JAK/STAT Pathway, PKA/PKC Pathway, WNT Signaling, Apoptosis/Autophagy, Cell Cycle, Cellular Stress, Angiogenesis, Invasion/Metastasis