Anti-Phospho-STAT5 (Tyr694)(S56-365BM)

Anti-Phospho-STAT5 (Tyr694)(S56-365BM)

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Description :Mouse Monoclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the mouse STAT5a protein phosphorylated at Tyrosine 694

Cited Applications :ELISA, IHC, IF, WB
Ideal working dilutions for each application should be empirically determined by the investigator.

Cross Reactivity :Human, Mouse and Rat

Host Isotype / Clone# :Mouse, IgG1

Immunogen :The antibody was produced against synthesized peptide corresponding to residues surrounding Tyr694 of mouse STAT5a protein.

Purification :Protein A chromatography

Stability :1yr at –20oC from date of shipment

Sample Data :Immunohistochemical analysis on formalin-fixed, paraffin embedded human breast tissues (40x) using Anti-Phospho-STAT5 (Tyr694) antibody.

Sample Data :Western blot using Anti-phospho-STAT5 (Tyr694) antibody (1:1,000) shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1).

Scientific Background :TAT5 (signal transducers and activators of transcription 5) is a member of the STAT family and this protein is phosphorylated by receptor associated protein kinases in response to cytokines and growth factors (1). The differentiation of T helper (Th) cells is regulated by members of the STAT family of signaling molecules. In BCR-ABL-positive cells, STAT5 is constitutively activated by tyrosine phosphorylation. STAT5 activation results in upregulation of Bcl-XL and increased resistance to induction of apoptosis. STAT5 is involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6 beta-hydroxylase (2).

References :
1. Wang, D. et al: Naturally occurring dominant negative variants of Stat5. Molec. Cell Biol. 16: 6141-6148, 1996.

2. A Subramanian et al: STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase. Nucleic Acids Res. 1998; 26(9): 2173–2178.

Product Sheets (By Lot #) :

B3216-83.pdf

Research Areas :Apoptosis/Autophagy, Cancer, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway