Anti-phospho-STAT3 (Ser727)(S54-365BR)

Anti-phospho-STAT3 (Ser727)(S54-365BR)

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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the GS3 beta protein phosphorylated at serine 727

Cited Applications :WB, IHC

Cross Reactivity :Human, Mouse and Rat

Host / Isotype / Clone# :Rabbit, IgG

Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser727

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blots from HeLa cells using anti-phospho- phospho-STAT3 (Ser727) antibody.

Sample Data :Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-STAT3 (Ser727) antibody.

Scientific Background :STAT3 is a member of the signal transducers and activators of transcription (STAT) family of proteins that carry out dual functions. STAT3 is a DNA binding protein that is widely expressed and becomes activated upon phosphorylation on Tyr708, which leads dimerization, intracellular translocation and DNA-binding. Phosphorylation at Ser727 regulates transcriptional activity. STAT3 is activated in response to variety of stimuli, which includes EGF, IL-6, PDGF, IL-2 and G-CSF (1). STAT3 forms homo- and hetero-dimers with other STAT proteins to modulate the transcription of various genes (2).

References :
1. Zhong, Z. et al: Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. Science. 1994 Apr 1;264(5155):95-8.

2. Tian, S. S. et al: Rapid activation of the STAT3 transcription factor by granulocyte colony-stimulating factor. Blood. 1994 Sep 15;84(6):1760.

Product Sheets (By Lot #) :

J1178-34.pdf

Research Areas :Apoptosis/Autophagy, Cancer, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway, Cancer, Inflammation, ERK/MAPK Pathway, JAK/STAT Pathway, Apoptosis/Autophagy