Anti-Phospho-Rel A (ser276)(R29-365R)

Anti-Phospho-Rel A (ser276)(R29-365R)

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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the human p65 protein phosphorylated at Serine 276

Cited Applications :ELISA, IHC, WB
Ideal working dilutions for each application should be empirically determined by the investigator

Cross Reactivity :Human

Host Isotype / Clone# :Rabbit, Antiserum

Immunogen :The antibody was produced against synthesized peptide corresponding to a region near phosphorylated Ser276 of the protein conjugated to KLH.

Purification :Delipidation and defibrination

Stability :1yr at –20oC from date of shipment

Sample Data :Western Blot of total protein lysates from head and neck tumors using anti-Phospho-Rel A (Ser276) antibody. Lanes 1-6: squamal carcinoma cells. Lane 7: primary culture of human keratinocytes Lane 8: ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9: EGF-induced human derived A431 cells.

Sample Data :Anti-pS529 shows phospho p65 staining in carcinoma cells.

Scientific Background :Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses In unstimulated cells, NF-κB is sequestered in the cytoplasm by its inhibitory proteins, the IκBs (1). NF-κB-activating agents can induce the phosphorylation of IκB’s, targeting them for rapid degradation through an ubiquitin-proteasome pathway, releasing NF-κB to enter the nucleus, where it regulates gene expression.

References :
1. Baeuerle, P. et al: Function and activation of NF-kappa B in the immune system. Annu Rev Immunol. 1994;12:141-79.

Product Sheets (By Lot #) :

B3216-59.pdf

Research Areas :Apoptosis/Autophagy, Cancer, Inflammation, Neurobiology, NfkB Pathway