Anti-phospho-RAF1 (Ser301)(R01-65R)

Anti-phospho-RAF1 (Ser301)(R01-65R)

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Description :Rabbit Polyclonal Antibody

Species :Rabbit

Tag :

Expression System:

Sequence :

Specificity :Recognizes the RAF1 protein phosphorylated at serine 301

Cited Applications :WB

Cross Reactivity :Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate and Xenopus

Host :Rabbit, IgG

Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser301 conjugated to KLH

Purification :Affinity Chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74kDa RAF1 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-RAF1 (Ser301) antibody. The immunolabeling of RAF1 is completely eliminated by treatment with lambda-Ptase.

Scientific Background :The Ras pathway is a critical signal transduction cascade that regulates cellular proliferation, differentiation, survival, and oncogenic transformation. The Ras family of membrane associated GTPases directly binds RAF1, its direct downstream effector (1). Activated RAF1 phosphorylates the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate the downstream serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2). Downregulation of RAF1 is regulated by phosphorylation at Ser301 and Ser642 (3).

References :
1. Rapp, U. et al: Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc. Nat. Acad. Sci. 80: 4218-4222, 1983.

2. Li, P. et al: Raf-1: a kinase currently without a cause but not lacking in effects. Cell 64: 479-482, 1991.

3. Dougherty M.K. et al: Regulation of Raf-1 by Direct Feedback Phosphorylation. Mol Cell 17:215-224, 2005.

Product Sheets (By Lot #) :

J1274-32.pdf

Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis, Ser/Thr Kinases