Anti-Phospho-MARK2 (Thr595)(M44-65R)

Anti-Phospho-MARK2 (Thr595)(M44-65R)

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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the human MARK2 protein phosphorylated at Threonine 595

Cited Applications :ELISA, IF, WB
Ideal working dilutions for each application should be empirically determined by the investigator.

Cross Reactivity :Human, Dog, Bovine, Mouse and Rat

Host Isotype / Clone# :Rabbit, IgG

Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids surrounding phosphorylated Thr595 of human MARK2 isoform a.

Purification :Immunoaffinity chromatography

Stability :1yr at –20oC from date of shipment.

Sample Data :Western Blot of Anti-Phospho-MARK2 (Thr595) (1:500 diltuion). Lane 1: wild type Jurkat cells. Lane 2: wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation). Load: 35 µg per lane.

Scientific Background :MARK2 or microtubule affinity-regulating kinase 2 is a member of the Par-1 family of serine/threonine protein kinases. MARK2 is an important regulator of cell polarity in epithelial and neuronal cells and controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins (1). MARK2 is a key target of H. pylori CagA in the disorganization of gastric epithelial architecture underlying mucosal damage, inflammation, and carcinogenesis (2). MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF13B to regulate axon formation. MARK2 function in the establishment of T cell polarity following engagement to an APC.

References :
1. Yoshimura, Y. et.al: Polarity-regulating kinase partitioning-defective 1/microtubule affinity-regulating kinase 2 negatively regulates development of dendrites on hippocampal neurons. . J. Neurosci. 27:13098-13107, 2010.

2. Saadat, I. et.al: Helicobacter pylori CagA targets PAR1/MARK kinase to disrupt epithelial cell polarity. Nature 447: 330-333, 2007.

Product Sheets (By Lot #) :

B3216-56.pdf

Research Areas :Invasion/Metastasis, Neurobiology, Ser/Thr Kinases