Anti-phospho-ERK1/2 (Thr202/Tyr204)(M29-652R)

Anti-phospho-ERK1/2 (Thr202/Tyr204)(M29-652R)

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Description :Rabbit Polyclonal Antibody

Species :Rabbit

Tag :

Expression System:

Sequence :

Specificity :Recognizes the ERK1/2 protein phosphorylated at threonine 202 and tryrosine 204

Cited Applications :WB, IHC

Cross Reactivity :Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Xenopus and Zebrafish

Host :Rabbit, IgG

Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH

Purification :Affinity Chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot of human T47D cell lysates showing specific immunolabeling of ~42-44kDa ERK1/2 protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-ERK1/2 (Thr202/Tyr204) antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Sample Data :Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK1/2 when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.

Scientific Background :Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is a serine threonine kinase. It plays an integral role of cellular signaling during mitogenesis and differentiation of mitotic cells. ERK is presumed to have a key role in learning and memory (1,2,3). The activity of this kinase is regulated by phosphorylation at Thr202 and Tyr204 (4). Activated ERK1/2 translocates into the nucleus where it phosphorylates various transcription factors (e.g Elk-1, c-Myc, c-Jun, c-Fos and C/EBP beta).

References :
1. Adams, J P. et al: Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 2002 42:135-163.

2. Johnson, G L. et al: Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 202 298:1911-1912.

3. Tanoue, T J. et al: Molecular recognitions in the MAP kinase cascades. Cellular Signaling 2003 15:455-462.

4. Ahn, N G.: The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 1993 127-128:201-209.

Product Sheets (By Lot #) :

J1274-16.pdf

Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis, Ser/Thr Kinases