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Anti-phospho-EPHB2 (Tyr473)(E22-65BR)

Anti-phospho-EPHB2 (Tyr473)(E22-65BR)

  • $385.00


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Description :Rabbit Polyclonal Antibody

Species :Rabbit

Tag :

Expression System:

Sequence :

Specificity :Recognizes the EPHB2 protein phosphorylated at tyrosine 473

Cited Applications :WB

Cross Reactivity :Human, Mouse, Bovine, Chicken, Xenopus and Zebrafish

Host :Rabbit, IgG

Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr473 conjugated to KLH

Purification :Affinity Chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot of rat testes lysate showing specific immunolabeling of the ~46kDa EPHB2 phosphorylated at Tyr473 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-EPHB2 (Tyr473). The immunolabeling of the EPHB2 is completely eliminated by treatment with lambda-Ptase.

Scientific Background :EPHB2 is a member of the Eph family of receptor tyrosine kinases that mediate neuro-developmental processes such as boundary formation, axon guidance, vasculogenesis and cell migration. Activated EPHB2 regulates the activity of the non-receptor tyrosine kinase Abl (1). EPHB2 is overexpressed in a number of tumors, particularly glioblastoma. In fact, EPHB2 overexpression leads to increased glioma cell migration and invasion (2).

References :
1. Yu, H H. et al: Multiple signaling interation of Abl and Arg kinases with the EPhB2 receptor. Oncogene. 2001 Jul 5;20(30):3995-4006.

2. Nakada, M. et al: The phosphorylation of EphB2 receptor regulates migration and invasion of human glioma cells. Cancer Res. 2004 May 1;64(9):3179-85.

3. Kalo, M S. et al: In vivo tyrosine phosphorylation sites of activated ephrin-B1 and ephB2 from neural tissue. J Biol Chem. 2001 Oct 19;276(42):38940-8.

Product Sheets (By Lot #) :

J1274-15.pdf

Research Areas :Angiogenesis, Cancer, Cardiovascular Disease, Neurobiology, Receptor Tyrosine Kinases, Cancer, Neurobiology, Cardiovascular Disease, Angiogenesis, Receptor Tyrosine Kinases