Anti-Phospho-Cyclin B1 (Ser126)(C79-365R)

Anti-Phospho-Cyclin B1 (Ser126)(C79-365R)

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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the human Cylcin B1 protein phosphorylated at Serine126

Cited Applications :ELISA, IP, WB
Ideal working dilutions for each application should be empirically determined by the investigator.

Cross Reactivity :Human, Rat, Dog, Chimpanzee, Mouse and Hamster

Host Isotype / Clone# :Rabbit, IgG

Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 12-131 of Human Cyclin B1 protein

Purification :Immunoaffinity chromatography followed by solid phase adsorption

Stability :1yr at –20oC from date of shipment

Sample Data :Western blot analysis using Anti-phospho-Cyclin B1 antibody (1:100) to detect phosphorylated human Cyclin B1 (~48kDa). lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). Each lane contains approximately 50 µg of lysates

Scientific Background :The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

References :
1. Giordano, A., et al.: A 60 kd cdc-2 associated polypeptide complexes with the E1A proteins of adenovirus-infected cells. Cell. 1989: 58; 981-990.

2. Pines, J., et al.: Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B. Nature. 1990: 346; 760-763.

Product Sheets (By Lot #) :

B3216-39.pdf

Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases