Anti-phospho-CREB1 (Ser133)(C49-565R)

Anti-phospho-CREB1 (Ser133)(C49-565R)

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Description :Rabbit Polyclonal Antibody

Species :Rabbit

Tag :

Expression System:

Sequence :

Specificity :Recognizes the CREB1 protein phosphorylated at serine 133

Cited Applications :WB

Cross Reactivity :Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Sheep, Xenopus and Zebrafish

Host :Rabbit, IgG

Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH

Purification :Affinity Chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot of forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45kDa CREB1 phosphorylated at Ser133 (Left lane). Phosphospecificity is shown in the right lane where the signal is completely eliminated by treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).

Sample Data :Immunostaining of cultured rat hippocampal slices showing nuclear labeling of CREB phosphorylated at Ser133 in brown.

Scientific Background :Members of the cAMP responsive element binding protein (CREB) family are critical mediators of gene expression in response to extracellular signals. Thus, they are essential regulators of adaptive behavior and long-term memory formation (1). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin dependent protein kinases (CAMKs) phosphorylate CRE1B at Ser133, which is required for CREB-mediated transcription (2,3). Activation of CREB1 has been implicated in the survival of mammalian cells. Mice that do not express CREB in the central nervous system display extensive apoptosis of postmitotic neurons during development (4). CREB can bind promoters containing CRE and activator protein 1 (AP-1) sites either as homo- and heterodimers to control gene expression.

References :
1. Valverde, O. et al: Modulation of anxiety-like behavior and morphine dependene in CREB-deficient mice. Neuropsychopharmacology. 2004 Jun;29(6):1122-33.

2. Johannessen, M. et al: What turns CREB on? Cellular Signalling 2004 16:1211-1227.

3. Kornhauser, J M. et al: CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 2002 34:221-233.

4. Mantamadiotis, T. et al: Disruption of CREB function in brain leads to neurodegeneration. Nat genet. 2002 May; 31(1): 47-54.

Product Sheets (By Lot #) :

J1274-12.pdf

Research Areas :Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, PKA/PKC Pathway, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy, Invasion/Metastasis