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Anti-phospho-ASK1 (Ser966)(M13-65R)

Anti-phospho-ASK1 (Ser966)(M13-65R)

  • $325.00


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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the ASK1 protein phosphorylated at serine 966

Cited Applications :IHC

Cross Reactivity :Human and Mouse

Host / Isotype / Clone# :Rabbit, IgG

Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser966

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-ASK1 (Ser966) antibody.

Scientific Background :ASK1, also known as MAPKKK5, is responsible for activating MKK3, MKK4 (SEK1), and MKK6. ASK1 is activated in cells treated with tumor necrosis factor-alpha and overexpression of ASK1 leads to increased apoptosis (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway leading to the activation of MKK4 and subsequently JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).

References :
1. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.

2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.

Product Sheets (By Lot #) :

J1178-2.pdf

Research Areas :Apoptosis/Autophagy, Cancer, Cellular Stress, JNK/SAPK Pathway, p38 Pathway, Ser/Thr Kinases, Cancer, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases