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Anti-phospho-AKT1/2/3 (Ser473)(A16-65BR)

Anti-phospho-AKT1/2/3 (Ser473)(A16-65BR)

  • $369.00


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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the AKT1/2/3 protein phosphorylated at serine 473

Cited Applications :WB, IHC

Cross Reactivity :Human, Mouse and Rat

Host / Isotype / Clone# :Rabbit, IgG

Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser473

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blots from HeLa cells treated with heat shock using AKT1/2/3 antibody (lanes 1 and 2) and anti-phospho-AKT1/2/3 (Ser473) antibody (lanes 3 and 4).

Sample Data :Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-phospho-AKT1/2/3 (Ser473) antibody.

Scientific Background :The AKT family of serine/threonine kinases includes AKT1, AKT2 and AKT3. They are activated in response to diverse stimuli such as hormones, growth factors and extracellular matrix components. AKTs are involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2). AKT is activated by phosphorylation at Thr308 and Ser473 by PDK1 and PDK2 respectively.

References :
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 369 ( Pt 1):1-13.

2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.

Product Sheets (By Lot #) :

J1178-30.pdf

Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling, Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, NfkB Pathway, WNT Signaling, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis, Ser/Thr Kinases