Anti-p21CIP1(C27-63R)

Anti-p21CIP1(C27-63R)

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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the p21CIP1 (CDKN1) protein

Cited Applications :WB

Cross Reactivity :Western blot human p21CIP1

p21CIP1 from other species may also be detectable.

Host  :Rabbit

Immunogen :Synthetic peptide corresponding to amino acids 143-147 of human p21CIP1

Purification :Affinity Chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative Western blot with Anti-p21CIP1 (1:1000) using 20ng of GST-tagged human recombinant p21CIP1.

Sample Data :Representative Western blot with Anti-p21CIP1 (1:1000) using 3 μg, 10 μg and 30 μg of THP1cell lysate.

Scientific Background :CIP1 (Cyclin-Dependent Kinase Inhibitor 1A) regulates cell cycle progression, terminal differentiation, and apoptosis (1). CIP1 was shown to be induced by p53 and to be a potent inhibitor of cyclin-dependent kinase (CDK) activity. DNA damage leads to increased expression of CIP1 in cyclin E-containing complexes and to an associated decrease in cyclin-dependent kinase activity. CIP1 is a critical downstream effector in the p53-specific pathway of growth control in mammalian cells (2).

References :
1. el-Deiry, W S. et al: WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994;54(5):1169-74

2. Wagner, M. et al: Cyclin-dependent kinase-inhibitor 1 (CDKN1A) in the squamous epithelium of the oropharynx: possible implications of molecular biology and compartmentation. Anticancer Res. 2001; 21(1A):333-45.

Product Sheets (By Lot #) :

M168-3.pdf

Research Areas :Cancer, Cell Cycle, Cancer, Cell Cycle