Anti-Myc(M86-61M)

Anti-Myc(M86-61M)

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Description :Mouse Monoclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Anti-Myc antibody recognizes the N-terminal or C-terminal Myc-tagged (EQKLISEEDL sequence) fusion protein.

Cited Applications :WB, ELISA, IP, IS

Cross Reactivity :All proteins tagged with Myc

Host / Isotype / Clone# :Mouse, IgG1, M19

Immunogen :EQKLISEED peptide conjugated to KLH

Purification :Purified by Protein A affinity chromatography from mouse ascites fluid

Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative western blot (1:1000) using in a positive cell lysate that contains Myc tagged fusion protein.

Scientific Background :The Myc epitope tag (EQKLISEEDL sequence) is highly charged and consists of ten amino acids derived from the Myc protooncogene. The cMyc protooncogene is a transcription factor regulating approximately 15% of genes, and is involved in cell proliferation, differentiation, and apoptosis.

References :
1. Jarvik, JW, and Telmer,CA. Epitope tagging, Annual Review of Genetics, 1998; 32, 601-618

2. Gearhart, J, et al: Advances in Stem-Cell Research, N Engl J Med, 2007; 357: 1469-1472

Product Sheets (By Lot #) :

L340-3.pdf

Research Areas :