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Description :Mouse Monoclonal Antibody
Specificity :Anti-Myc antibody recognizes the N-terminal or C-terminal Myc-tagged (EQKLISEEDL sequence) fusion protein.
Cited Applications :WB, ELISA, IP, IS
Cross Reactivity :All proteins tagged with Myc
Host / Isotype / Clone# :Mouse, IgG1, M19
Immunogen :EQKLISEED peptide conjugated to KLH
Purification :Purified by Protein A affinity chromatography from mouse ascites fluid
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot (1:1000) using in a positive cell lysate that contains Myc tagged fusion protein.
Scientific Background :The Myc epitope tag (EQKLISEEDL sequence) is highly charged and consists of ten amino acids derived from the Myc protooncogene. The cMyc protooncogene is a transcription factor regulating approximately 15% of genes, and is involved in cell proliferation, differentiation, and apoptosis.
1. Jarvik, JW, and Telmer,CA. Epitope tagging, Annual Review of Genetics, 1998; 32, 601-618
2. Gearhart, J, et al: Advances in Stem-Cell Research, N Engl J Med, 2007; 357: 1469-1472
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