FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the mTOR protein
Cited Applications :WB
Cross Reactivity :Western blot of mTOR from human cells
mTOR from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to amino acids 2446-2700 of human mTOR
Purification :Affinity chromatography
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-mTOR (1:1000) using 3 μg, 10 μg, and 30 μg of HeLa cell lysate.
Scientific Background :The mammalian target of rapamycin, mTOR, also known as FRAP or RAFT, is a Ser/Thr protein kinase. mTOR acts as a sensor for ATP and amino acids, balancing the availability of nutrients and cell growth (1). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal, transmits a positive signal to p70 S6 kinase and participates in the inactivation of the eIF4E inhibitor, 4E-BP1 (2).
1. Sabers CJ et al: Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells. J Biol Chem. 270(2): 815-22, 1995.
2. Fang Y. et al: Phosphatidic acid-mediated mitogenic activation of mTOR signaling. Science. 294(5548): 1942-5, 2001.
Product Sheets (By Lot #) :
Research Areas :Cancer, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, Apoptosis/Autophagy, Angiogenesis, Cancer, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, Apoptosis/Autophagy, Angiogenesis