Anti-Methylated Arginine (NG, NG-Di-methyl)(A96-67R)
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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes proteins with NG, NG-di-methylated arginine residues.
Cited Applications :WB, ELISA, IF, IHC, IP
Cross Reactivity :Pan-specific antibody. No cross-reaction with acetylated proteins and mono-methylated proteins.
Immunogen :NG, NG-di-methylated arginine-KLH conjugates
Purification :By affinity chromatography on a di-methyl arginine agarose column
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot of the di-methylated arginine protein profile with Anti-Methylated Arginine (NG, NG-Di-methyl) (1:1000) using human melanoma (MMRU) cell lysate (A) and the MMRU lysate plus additional di-methyl arginine peptide inhibitor (B).
Sample Data :Immunofluorecent stain of human melanoma (MMRU) cell with Anti-Methylated Arginine (NG,NG-Di-methyl).
Scientific Background :Post-translational modification of proteins and peptides is a robust way to regulate function of existing proteins or peptides. Methylation on arginine residues is one example of post-translational modification and is performed by a variety of protein methyl transferases in the cell. Arginine methylation of Rad9 for example is performed by protein arginine methyltransferase 5 (PRMT5) is important for regulation of Rad9 function and is a major element for maintaining genome integrity (1). The Methylated Arginine antibody detects methylation on arginine residues in proteins and peptide.
1. He, W. et al: A role for the arginine methylation of Rad9 in checkpoint control and cellular sensitivity to DNA damage. Nucleic Acids Res. 2011 Feb 14.
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Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Cancer, Apoptosis/Autophagy, Cell Cycle