Anti-Methylated Arginine (NG-Mono-methyl)(A95-67R)

Anti-Methylated Arginine (NG-Mono-methyl)(A95-67R)

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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes proteins with NG-mono-methylated arginine residues.

Cited Applications :WB, ELISA, IF, IHC, IP

Cross Reactivity :Pan-specific antibody. No reaction to acetylated proteins, di-methylated and tri-methylated proteins.

Host :Rabbit

Immunogen :NG-mono-methylated arginine-KLH conjugates

Purification :By affinity chromatography on a mono-methyl arginine agarose column

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative western blot of the mono-methylated arginine protein profile with Anti-Methylated Arginine (NG-Mono-methyl) (1:1000) using human melanoma (MMRU) cell lysate (A) and the MMRU lysate plus additional mono-methyl arginine peptide inhibitor (B).

Sample Data :Immunofluorecent stain of human melanoma (MMRU) cell with Anti-Methylated Arginine (NG-Mono-methyl).

Scientific Background :Post-translational modification of proteins and peptides is a robust way to regulate function of existing proteins or peptides. Methylation on Arginine residues is one example of post-translational modification and is performed by a variety of protein methyl transferases in the cell. Arginine methylation of Rad9 for example is performed by protein arginine methyltransferase 5 (PRMT5) is important for regulation of Rad9 function and is a major element for maintaining genome integrity (1). The Methylated Arginine antibody detects Methylation on Arginine residues in proteins and peptide.

References :
1. He, W. et al: A role for the arginine methylation of Rad9 in checkpoint control and cellular sensitivity to DNA damage. Nucleic Acids Res. 2011 Feb 14.

Product Sheets (By Lot #) :

E360-2.pdf

Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Cancer, Apoptosis/Autophagy, Cell Cycle