Anti-MARCKS(M02-562R)

Anti-MARCKS(M02-562R)

  • $330.00


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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the MARCKS protein

Cited Applications :WB, IF

Cross Reactivity :Rat

Host :Rabbit, IgG

Immunogen :Recombinant protein expressed in and purified from E. coli

Purification :Neat Serum

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot of rat cortex lysate showing specific immunolabeling of the ~80kDa MARCKS protein.

Sample Data :Mixed neuron/glial cultures stained with the Anti-MARCKS antibody (red) anti-MAP2 (green).

Scientific Background :Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a member of a family of calmodulin binding proteins that isa target for phosphorylation by protein kinase C (PKC) (1). MARCKS is concentrated in the synapses of neurons where it appears to function in synaptic vesicle cycling. MARCKS has been shown to bind both actin and calmodulin in vitro (2). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (3). Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (4).

References :
1. Ouimet, C C. et al: Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. 1990 J Neurosci 10:1683-1698.

2. Hartwig J H. et al: MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 1993356: 618-622.

3. Stumpo D J. et al: MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 1995 92; 944-948.

4. Verghese G M. et al: Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem 1994 269:9361-9367.

Product Sheets (By Lot #) :

J1274-18.pdf

Research Areas :Neurobiology, Invasion/Metastasis, Ser/Thr Kinases