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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the KDM1A protein
Cited Applications :WB, ELISA, IF, IHC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :KDM1A antibody was raised against a 16 amino acid synthetic peptide from near the center of human KDM1A
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of KDM1A in P815 cell lysate with KDM1A antibody at (A) 1 and (B) 2 ug/ml.
Sample Data :Immunohistochemistry of KDM1A in human small intestine tissue with KDM1A antibody at 2 ug/ml.
Scientific Background :KDM1A or lysine (K)-specific demethylase 1A is a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain that is a component of several histone deacetylase complexes and act as a histone demethylase. (1) H3K4 histone demethylase activity of KDM1A is partly responsible for the repressive activity of TAL1 and restricts TAL1 function in hematopoiesis (1). KDM1A plays an essential role for CoREST in demethylation of H3K4 both in vitro and in vivo (2).
1. Hu, X. et.al : LSD1-mediated epigenetic modification is required for TAL1 function and hematopoiesis. Proc. Nat. Acad. Sci. 106: 10141-10146, 2009.
2. Lee, M. G. et.al : An essential role for CoREST in nucleosomal histone 3 lysine 4 demethylation. Nature 437: 432-435, 200.
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Research Areas :Cancer, Cell Cycle, Inflammation, Cancer, Inflammation, Cell Cycle