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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the JNK1 protein
Cited Applications :WB
Cross Reactivity :Western blot JNK1 from human, mouse, and rat cells
JNK1 from other species may also be detectable.
Immunogen :Protein Code: P45983
Purification :Affinity purified by the peptide antigen
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Western Blot :
Representative western blot with Anti-JNK1 (1:250) using 20 ng of JNK1, JNK2, and JNK3 recombinant protein.
Scientific Background :JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at thr and tyr residues during exposure to stress such as UV irradiation. JNK1 binds to the c-Jun transactivation domain and phosphorylates it on Ser-63 and Ser-73 (1). JNK1 has been shown to play an important role in disease processes. Activation of JNK1 results in defects in myotube viability and integrity leading to dystrophic myofiber destruction (2). JNK1 activity is also abnormally elevated in obesity and removal of JNK1 results in decreased adiposity and significantly improved insulin sensitivity.
1. Derijard, B. et al: JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell. 1994 Mar 25;76(6):1025-37.
2. Kolodziejczyk, S M. et al: Activation of JNK1 contributes to dystrophic muscle pathogenesis. Curr Biol. 2001 Aug 21;11(16):1278-82.
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Research Areas :Neurobiology, Inflammation, Cardiovascular Disease, JNK/SAPK Pathway, NfkB Pathway, Apoptosis/Autophagy, Cellular Stress, Neurobiology, Inflammation, Cardiovascular Disease, JNK/SAPK Pathway, NfkB Pathway, Apoptosis/Autophagy, Cellular Stress