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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the JAK2 protein
Cited Applications :WB
Cross Reactivity :Western blot JAK2 from human and mouse cells
JAK2 from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to amino acids 1005-1009 of human JAK2
Purification :Affinity chromatography
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-JAK2 (1:1000) using 20 ng of human recombinant JAK1 and JAK2.
Sample Data :Representative western blot with Anti-JAK2 (1:1000) using 30 μg of HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysate.
Scientific Background :JAK2 is a member of intracellular non-receptor tyrosine kinases that transduce cytokine-mediated signals via the JAK-STAT pathway (1). JAK2 has two near-identical phosphate-transferring domains. One domain exhibits the kinase activity while the other stabilizes the JAK conformational structure. JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin. JAK2 has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon (2).
1. Lacronique, V. Et al : A TEL-JAK2 fusion protein with constitutive kinase activity in human leukemia. Science, 1997; 278: 1309-1312.
2. Ho, J.M. et al: TEL-JAK2 constitutively activates the extracellular signal-regulated kinase (ERK), stress-activated protein/Jun kinase (SAPK/JNK), and p38 signaling pathways. Blood, 2002; 100: 1438-1448.
Product Sheets (By Lot #) :
Research Areas :Cancer, Inflammation, Cardiovascular Disease, JAK/STAT Pathway, Cytoplasmic Tyrosine Kinases, Cancer, Inflammation, Cardiovascular Disease, JAK/STAT Pathway, Cytoplasmic Tyrosine Kinases