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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the IRAK4 protein and has no cross response to other IRAKs
Cited Applications :WB, ELISA, ICC, IF
Cross Reactivity :Human
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :IRAK4 antibody was raised against a synthetic peptide corresponding to amino acids at the carboxy terminus of human IRAK4
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of IRAK4 in HeLa cell lysate with IRAK4 antibody at 1 (lane 1), 2 (lane 2), and 4 (lane 3) ug/ml, respectively.
Sample Data :Immunocytochemistry of IRAK4 in K562 cells with IRAK4 antibody at 10 ug/ml.
Scientific Background :Interleukin-1 receptor-associated kinase 4 (IRAK4) is an important mediator in the signal transduction of Toll-like receptor (TLR) and IL1R family members (1). IRAK4 is involved in the Toll-like receptor signaling pathway leading to Apoptosis. IRAK4 has molecular functions like protein binding, ATP binding, kinase activity and magnesium ion binding. Toll/IL-1 receptor family members like IRAK4 are central components of host defense mechanisms in a variety of species (2). One well conserved element in their signal transduction is the Ser/Thr kinase activity which couple early signaling events in a receptor complex at the plasma membrane to larger signalosomes in the cytosol.
1. Li, S. et al: IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase. Proc. Natl. Acad. Sci. USA. 2002; 99:5567-72.
2. Suzuki, N. et al: IRAK-4 as the central TIR signaling mediator in innate immunity. Trends Immunol. 2002; 23:503-6.
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Research Areas :Inflammation, NfkB Pathway, p38 Pathway, Ser/Thr Kinases, Inflammation, p38 Pathway, NfkB Pathway, Ser/Thr Kinases