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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the IL-21 protein
Cited Applications :WB, ELISA, ICC
Cross Reactivity :Human
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :Anti-IL-21 antibody was raised against a synthetic peptide corresponding to 15 amino acids near the center of human IL-21 precursor.
Formulation :PBS containing 0.02% sodium azide.
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with Anti-IL-21 antibody at 1 ug /ml.
Sample Data :Immunocytochemistry of IL-21 in K562 cells with Anti-IL-21 antibody at 2 ug/mL.
Scientific Background :A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gamma c, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
1. Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature. 2000; 408(6808):57-63.
2. Asao H, Okuyama C, Kumaki S, et al. the common γ-chain is an indispensable subunit of the IL-21 receptor complex. J Immunol. 2001;167(1):1-5.
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Research Areas :Cancer, Inflammation, Cardiovascular Disease, JAK/STAT Pathway, Cytoplasmic Tyrosine Kinases