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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the IKK gamma protein and has no cross response to IKK alpha or IKK beta
Cited Applications :WB, ELISA, ICC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :IKK gamma antibody was raised against a peptide corresponding to amino acids 400 to 416 of human IKK gamma, which are identical to those of mouse homologue.
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 ug/ml.
Sample Data :Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 ug/ml.
Scientific Background :IKKgamma is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. IKKgamma forms dimers and trimers and interacts preferentially with IKKbeta but not IKKalpha (1). IKKgamma associates with activated ATM after the induction of DNA double-strand breaks (2). ATM phosphorylates serine-85 of IKKgamma to promote its ubiquitin-dependent nuclear export.
1. Rothwarf, D. M. et al: IKK-gamma is an essential regulatory subunit of the I-kappa-B kinase complex. Nature 395: 297-300, 1998.
2. Wu, Z-H. et al: Molecular linkage between the kinase ATM and NF-kappaB signaling in response to genotoxic stimuli. Science 311: 1141-1146, 2006.
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Research Areas :Cancer, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling, Inflammation, AKT/PKB Pathway, p38 Pathway, Apoptosis/Autophagy