Anti-IKK alpha(C51-63R)

Anti-IKK alpha(C51-63R)

  • $325.00


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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the IKK alpha protein with no cross response to IKKb or IKKg

Cited Applications :WB, ELISA, ICC, IF

Cross Reactivity :Human

Host / Isotype / Clone# :Rabbit, IgG

Immunogen :IKK alpha antibody was raised against a peptide corresponding to amino acids 716 to 734 of human IKK alpha, which differs from corresponding murine sequence by four amino acids.

Formulation :PBS + 0.02% sodium azide.

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:1000 dilution.

Sample Data :Immunocytochemistry of IKK alpha in Jurkat cells with IKK alpha antibody at 1ug/ml.

Scientific Background :IKKalpha is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex (1). IKKalpha is an essential regulator of NF-kappa-B-dependent gene expression through control of promoter-associated histone phosphorylation after cytokine exposure (2). IKKalpha is a critical component of the cytoplasmic transductional-transcriptional processor leading to induction of IFNalpha production. IKKalpha is also involved in the epidermis where it antagonizes mitogenic and angiogenic signals and represses tumor progression and metastases.

References :
1. Anest, V.et.al: A nucleosomal function for I-kappa-B kinase-alpha in NF-kappa-B-dependent gene expression. Nature 423: 659-663, 2003.

2. Hoshino, K. et.al: I-kappa-B kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9. Nature 440: 949-953, 2006.

Product Sheets (By Lot #) :

O2109-6.pdf

Research Areas :Cancer, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling, Inflammation, AKT/PKB Pathway, p38 Pathway, Apoptosis/Autophagy