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Description :Mouse Monoclonal Antibody
Specificity :Recognizes the HSP27 protein
Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator
Cross Reactivity :Human
Host Isotype / Clone# :Mouse, IgG1
Immunogen :The antibody was produced against prokaryotic recombinant protein corresponding to the full length human HSP27 protein
Formulation :0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 + 0.01% (w/v) Sodium Azide
Purification :Protein A Chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Western Blot using Anti-HSP27 (1:1000) antibody to detect human HSP27. 10 µg of HeLa whole cell lysate loaded per lane
Scientific Background :HSP27 is a member of heat shock proteins (HSPs) which are synthesized in cells in response to heat shock and other metabolic stresses and provide a transient state of thermotolerance. HSP27 plays a major role in the increased thermal resistance acquired by cells after exposure to HSP inducers (1). The level of HSP27 phosphorylation is significantly elevated after exposure of cells to heat shock, sodium arsenite, IL-1 and TNF-α. MAPKAPK2 and MAPKAPK3 are both activated by these conditions and can phosphorylate HSP27 on serine residues (2).
1. Landry, J. et al: Heat shock resistance conferred by expression of the human HSP27 gene in rodent cells. J Cell Biol. 1989 Jul: 109(1); 7-15.
2. Clifton, A D. et al: A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress. FEBS Lett. 1996: 392(3); 209-14.
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Research Areas :Cancer, Cellular Stress, p38 Pathway