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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the human Histone 3 protein
Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Mouse, Rat, Chicken, Dog, Monkey, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans and fruit fly
Host Isotype / Clone# :Rabbit, antiserum
Immunogen :The antibody was produced against synthesized peptide corresponding to the C-Terminus region of human histone 3
Formulation :0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 + 0.01% (w/v) Sodium Azide
Purification :Delipidation and defibrination
Stability :1yr at –20oC from date of shipment
Sample Data :Immunohistochemical analysis of formalin fixed, paraffin embedded lung tissues using anti-histone H3 antibody (1:100)
Sample Data :Western Blot of Anti-Histone 3 antibody (1:2000). Lane 1: Raji lysate. Lane 2: HeLa lysate. Load: 15ug, 25ug respectively.
Scientific Background :Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone proteins are highly post-translationally modified with Histone H3 being the most extensively modified of the five histones. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 Antibody is ideal for investigators involved in Cell Signaling, Epigenetics, Nuclear Signaling research and Signal Transduction research.
1. Peterson, C. L., et al.: Histones and histone modifications. Current Biology. 2004: 14(14); R546-R551. doi:10.1016/j.cub.2004.07.007.
2. Hake, S. B., et al.: Histone H3 variants and their potential role in indexing mammalian genomes: the "H3 barcode hypothesis". Proceedings of the National Academy of Sciences of the United States of America. 2006: 103(17); 6428-6435, doi:10.1073/pnas.0600803103.
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Research Areas :Cancer, Cell Cycle, ERK/MAPK Pathway, PKA/PKC Pathway