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Description :Mouse Monoclonal Antibody
Specificity :Anti-His antibody recognizes the N-terminal, C-terminal or internal 6X His-tagged fusion protein.
Cited Applications :WB, ELISA, IP, IS
Cross Reactivity :All proteins tagged with 6X His
Host / Isotype / Clone# :Mouse, IgG2b, EH158
Immunogen :HHHHHH peptide conjugated to KLH
Formulation :10mM PBS, pH 7.2
Purification :Purified by Protein A affinity chromatography from mouse ascites fluid
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot (1:1000) using in a positive cell lysate that contains His tagged fusion protein.
Scientific Background :This polyhistidine epitope tag is generally comprised of six consecutive histidine amino acid residues located at the N-terminal, C-terminal, or internally . The 6 His-Tag is widely used because of its affinity to bind nickel or cobalt metal ions attached to sepharose, which can then be used to purify the protein in a native or denatured state .
1. Jonathan, W. et al: Epitope tagging, Annual Review of Genetics, 1998; 32, 601-618
2. Schmeisser, H, et al:, Binding Characteristics of IFN-alpha Subvariants to IFNAR2-EC and Influence of the 6-Histidine Tag. J Interferon Cytokine Res. 2006; Dec. 26(12):866-76.
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