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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the HER3 protein
Cited Applications :ELISA, IP, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Mouse and Rat
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against HER3 fusion protein corresponding to amino acids 1283 and 1323 at the C-terminus of human HER3
Formulation :0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 + 0.01% (w/v) Sodium Azide
Purification :Protein A Chromatography followed by cross adsorption against GST and extensive dialysis
Stability :1yr at –20oC from date of shipment
Sample Data :Combined immunoprecipitation and western blot of a human cell lysate using Anti-HER3 antibody. Lysates were prepared from a human LNCap prostate cell line both with (+) treatment with Heregulin-b1 for 15' at 100 ng/ml and without (-) the addition of Heregulin-b1.
Scientific Background :The widely expressed EGFR receptor tyrosine kinase family consists of 4 members: EGFR, HER2, HER3 and HER4. These kinases share up to 70% sequence homology in their extracellular domains. Signaling involves the combinatorial dimerization of the four known family members and is mediated by at least 8 different hormones, including EGF and the heregulins. Heterodimerization of HER2 with HER3 or HER4 enhances the HER2’s ability to bind Heregulin, leading to enhanced downstream tyrosine phosphorylation. These RTKs are implicated in many human cancers and strong concurrent expression of HER3 and HER2 leads to poor outcomes.
1. Vijapurkar, U., et al.: The ErbB3/HER3 receptor protein: signaling mechanisms and biologic function. Curr. Top. Biochem. Res. 1999; 1 : 37-57
2. Zanazzi G, et al.: Glial growth factor/neuregulin inhibits schwann cell myelination and induces demyelination. J Cell Biol. 2001: 152(6):1289-300.
3. Liu Z, et al.: Expression of the receptor tyrosine kinases, epidermal growth factor receptor, ErbB2, and ErbB3, in human ocular surface epithelia. Cornea. 2001: 20(1):81-5.
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Research Areas :AKT/PKB Pathway, Cancer, ERK/MAPK Pathway, Receptor Tyrosine Kinases