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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the HER2 protein
Cited Applications :WB, ELISA, IF, IHC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :HER2 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human HER2
Formulation :PBS + 0.02% sodium azide.
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of HER2 in rat liver tissue lysate with HER2 antibody at (A) 1 and (B) 2 ug/ml.
Sample Data :Immunohistochemistry of HER2 in human liver tissue with HER2 antibody at 2.5 ug/ml.
Scientific Background :HER2 gene encodes a cell-surface glycoprotein tyrosine kinase receptor with extensive homology to the epidermal growth factor receptor. HER2 is an oncogene and overexpression of unaltered HER2 coding sequences in NIH 3T3 cells results in cellular transformation and tumorigenesis (1). HER2 is amplified in about 30% of primary human breast malignancies and overexpression of HER2 is associated with the most aggressive tumors that show uncontrolled proliferation, resistance to apoptosis and increased motility (2).
1. Hudziak, R M. et al: Increased expression of the putative growth factor receptor p185HER2 causes transformation and tumorigenesis of NIH 3T3 cells. Proc Natl Acad Sci U S A. 1987 Oct; 84(20):7159-63.
2. Badache, A. et al: The ErbB2 Signaling Network as a Target for Breast Cancer Therapy. J Mammary Gland Biol Neoplasia. 2006 Jan; 11(1):13-25.
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Research Areas :AKT/PKB Pathway, Cancer, Receptor Tyrosine Kinases, Cancer, AKT/PKB Pathway, Receptor Tyrosine Kinases