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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the human HDAC5 protein
Cited Applications :WB
Cross Reactivity :Only a weak reaction with human HDAC4 in Western Blot.
HDAC5 from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to c-terminus of human HDAC5 conjugated to KLH.
Purification :Affinity Chromatography
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-HDAC5 (1:1000) using 25 ng of human recombinant proteins HDAC1 to HDAC11.
Scientific Background :HDAC5 or Histone deacetylase 5 belongs to the class II histone deacetylase/acuc/apha family that possesses histone deacetylase activity and represses transcription when tethered to a promoter (1). HDAC 5 plays a critical role in transcriptional regulation, cell cycle progression, and developmental events and also acts as a potential therapeutic target for the prevention of atherosclerosis. HDAC5 can co-immunoprecipitates with HDAC3 family members forming multicomplex proteins. HDAC5 can also interact with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. HDAC5 gene is thought to be associated with colon cancer (2).
1. Grozinger, C. M. et.al : Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc. Nat. Acad. Sci. 96: 4868-4873, 1999.
2. Scanlan, M. J. et.al: Characterization of human colon cancer antigens recognized by autologous antibodies. Int. J. Cancer 76: 652-658, 1998.
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Research Areas :Cardiovascular Disease, WNT Signaling, Cardiovascular Disease, WNT Signaling