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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the HDAC5 protein
Cited Applications :ELISA, IHC, IF, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to the internal region surrounding phosphoserine 661 of human HDAC5
Formulation :0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 + 50% (v/v) Glycerol
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Western Blot of Anti-HDAC5 antibody (0.2µg/mL). Lane 1: mouse brain extract. Load: 5 µg per lane.
Scientific Background :HDAC5 or Histone deacetylase 5 belongs to the class II histone deacetylase/acuc/apha family that possesses histone deacetylase activity and represses transcription when tethered to a promoter (1). HDAC 5 plays a critical role in transcriptional regulation, cell cycle progression, and developmental events and also acts as a potential therapeutic target for the prevention of atherosclerosis. HDAC5 can co-immunoprecipitates with HDAC3 family members forming multicomplex proteins. HDAC5 can also interact with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. HDAC5 gene is thought to be associated with colon cancer (2).
1. Grozinger, C. M. et.al : Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc. Nat. Acad. Sci. 96: 4868-4873, 1999.
2. Scanlan, M. J. et.al: Characterization of human colon cancer antigens recognized by autologous antibodies. Int. J. Cancer 76: 652-658, 1998.
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Research Areas :Cancer, Cell Cycle, Inflammation