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Description :Mouse Monoclonal Antibody
Specificity :Anti-GST antibody recognizes the N-terminal or C-terminal GST-tagged fusion protein.
Cited Applications :WB
Cross Reactivity :All proteins tagged with GST
Host / Isotype / Clone# :Mouse, IgG2a, G2R
Immunogen :Purified GST
Formulation :10mM PBS, pH 7.2
Purification :Purified by Protein A affinity chromatography from mouse ascites fluid
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot (1:1000) using GST tagged fusion protein.
Scientific Background :GST or glutathione S-transferase family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are used to create the so-called 'GST gene fusion system' (1). These proteins are capable of multiple reactions with a multitude of substrates. GST tag has the size of 220 amino acids, which is relatively big compared to other tags like the Myc- or the DYKDDDDK-tag that are quite small. GST can be used to purify and detect proteins of interest.
1. Beckett, G.J. and Hayes, J.D. Glutathione S-transferase measurements and liver disease in man. J. Clin. Biochem. Nutr. 1987. 2, 1-24.
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