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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the GSK3α protein
Cited Applications :WB
Cross Reactivity :Western blot of human GSK3a
GSK3a from other species may also be detectable.
Immunogen :Protein Code P49840
Purification :Affinity Chromatography
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot (1:1000) with Anti-GSK3α using 20 ng of GST-tagged human recombinant GSK3α and GSK3β.
Scientific Background :GSK3α is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase and transcription factors (e.g., JUN) (1). GSK3α also plays a role in the WNT and PI3K signaling pathways. Alzheimer disease is associated with increased production and aggregation of amyloid-beta-40 and -42 peptides into plaques. GSK3α is required for maximal production of the beta-amyloid-40 and -42 peptides generated from the amyloid precursor protein by presenilin-dependent gamma-secretase cleavage. In vitro, lithium, a GSK3α inhibitor, blocks the production of the beta-amyloid peptides by interfering with the gamma-secretase step (2).
1. Ali, A. et al: Glycogen synthase kinase-3 : properties, functions, and regulation. Chem. Rev. 101: 2527-2540, 2001.
2. Phiel, C J. et al: GSK-3-alpha regulates production of Alzheimer's disease amyloid-beta peptides. Nature 423: 435-439, 2003.
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Research Areas :Neurobiology, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, Ser/Thr Kinases, Neurobiology, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, Ser/Thr Kinases