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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the GSK3α and GSK3β protein
Cited Applications :WB
Cross Reactivity :Western blot of GSK3a and GSK3B from human cells
GSK3a and GSK3B from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to amino acids 214-218 of human GSK3β
Purification :Affinity chromatography
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-GSK3α/β (1:1000) using 20 ng of GST tagged human recombinant GSK3α and GSK3β.
Sample Data :Representative western blot with Anti-GSK3 (1:1000) using 30 μg of HeLa, HEK-293T, and Jurkat cell lysate.
Scientific Background :GSK3α is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase and transcription factors (e.g., JUN) (1). GSK3β is ubiquitously present in human tissues and implicated in the regulation of several physiological processes, including the control of glycogen and protein synthesis by insulin, modulation of the transcription factors AP-1 and CREB (2).
1. Ali, A. et al: Glycogen synthase kinase-3 : properties, functions, and regulation. Chem. Rev. 101: 2527-2540, 2001.
2. Sperber BR, Leight S, Goedert M, Lee VM. Glycogen synthase kinase-3 beta phosphorylates tau protein at multiple sites in intact cells. Neurosci Lett. 1995 Sep 8;197(2):149-53.
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Research Areas :Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, JNK/SAPK Pathway, PKA/PKC Pathway, WNT Signaling, Angiogenesis, Invasion/Metastasis, Neurobiology, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, Ser/Thr Kinases