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Description :Rabbit Polyclonal Antibody
Specificity :Recognizes the GATA1 protein
Cited Applications :WB
Cross Reactivity :Western blot human and mouse GATA1
GATA1 from other species may also be detectable.
Immunogen :Synthetic peptide corresponding to amino acids 308-312 of human GATA1
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative Western blot with Anti-GATA1 (1:1000) using 20 μg of Hela, 293, Jurkat, THP1 and 3T6 cell lysate.
Scientific Background :GATA1 is a member of the GATA family of transcription factors and plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. FOG, a zinc finger protein, interacts with the amino (N) finger of GATA1 and this interaction is essential for the function of GATA1 in erythroid differentiation (1). Caspase-mediated cleavage of GATA1 is an important negative control mechanism in erythropoiesis. Mutations in GATA1 have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.
1. Crispino, J D. et al: Use of altered specificity mutants to probe a specific protein-protein interaction in differentiation: the GATA-1:FOG complex. Molec. Cell 3: 219-228, 1999.
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Research Areas :Cancer, Neurobiology, Cancer, Neurobiology